Trehalose Is A Chemical Attractant In The Establishment Of Coral Symbiosis

Coral reefs have evolved with a crucial symbiosis between photosynthetic dinoflagellates (genus Symbiodinium) and their cnidarian hosts (Scleractinians). Most coral larvae take up Symbiodinium from their environment; however, the earliest steps in this process have been elusive. Here we demonstrate that the disaccharide trehalose may be an important signal from the symbiont to potential larval hosts. Symbiodinium freshly isolated from Fungia scutaria corals constantly released trehalose (but not sucrose, maltose or glucose) into seawater, and released glycerol only in the presence of coral tissue. Spawning Fungia adults increased symbiont number in their immediate area by excreting pellets of Symbiodinium, and when these naturally discharged Symbiodinium were cultured, they also released trehalose. In Y-maze experiments, coral larvae demonstrated chemoattractant and feeding behaviors only towards a chamber with trehalose or glycerol. Concomitantly, coral larvae and adult tissue, but not symbionts, had significant trehalase enzymatic activities, suggesting the capacity to utilize trehalose. Trehalase activity was developmentally regulated in F. scutaria larvae, rising as the time for symbiont uptake occurs. Consistent with the enzymatic assays, gene finding demonstrated the presence of a trehalase enzyme in the genome of a related coral, Acropora digitifera, and a likely trehalase in the transcriptome of F. scutaria. Taken together, these data suggest that adult F. scutaria seed the reef with Symbiodinium during spawning and the exuded Symbiodinium release trehalose into the environment, which acts as a chemoattractant for F. scutaria larvae and as an initiator of feeding behavior- the first stages toward establishing the coral-Symbiodinium relationship. Because trehalose is a fixed carbon compound, this cue would accurately demonstrate to the cnidarian larvae the photosynthetic ability of the potential symbiont in the ambient environment. To our knowledge, this is the first report of a chemical cue attracting the motile coral larvae to the symbiont

Noise in Electron Devices

Contains research objectives and reports on one research project.Lincoln Laboratory, Purchase Order DDL B-00368U. S. Air Force under Air Force Contract AF19(604)-7400U. S. NavyU. S. Arm

Noise in Electron Devices

Contains research objectives and reports on two research projects

Microwave and Millimeter Wave Techniques

Contains reports on one research project.Joint Services Electronics Program (Contract DAAB07-71-C-0300

Electromagnetic self-forces and generalized Killing fields

Building upon previous results in scalar field theory, a formalism is developed that uses generalized Killing fields to understand the behavior of extended charges interacting with their own electromagnetic fields. New notions of effective linear and angular momenta are identified, and their evolution equations are derived exactly in arbitrary (but fixed) curved spacetimes. A slightly modified form of the Detweiler-Whiting axiom that a charge's motion should only be influenced by the so-called "regular" component of its self-field is shown to follow very easily. It is exact in some interesting cases, and approximate in most others. Explicit equations describing the center-of-mass motion, spin angular momentum, and changes in mass of a small charge are also derived in a particular limit. The chosen approximations -- although standard -- incorporate dipole and spin forces that do not appear in the traditional Abraham-Lorentz-Dirac or Dewitt-Brehme equations. They have, however, been previously identified in the test body limit.Comment: 20 pages, minor typos correcte

Electrodynamics of Media

Contains research objectives and reports on four research projects.Joint Services Electronics Programs (U. S. Army, U.S. Navy, and U.S. Air Force) under Contract DA 28-043-AMC-02536(E